Equipment

• X-ZELL Automated Cryostaining Suite (7 color-fluorescence in cryosections)
• Nanostring Sprint (no prep-station)
• LEICA microscope with LEICA Thunder 3D Imaging Software:

  label microscope	channels	filter	filter	filter
  421	BV421/DAPI/AF405	395/25	431/28	412Ip
  480	BV480	436/20	480/30	455Ip
  488	AF488 FITC	495/25	537/29	515Ip
  PE	PE, propidium iodide	546/10	537/29	556Ip
  594	AF594	599/13	632/28	612Ip
  DRQ	DRAQ5/AF647	642/20	697/60	660Ip
  PER	PerCP*	445/30	697/60	510Ip

  *bleaches quickly

The following material has to be provided by the user:

• X-Zell Cryoimmunostaining Kit

Cactus Biotec (art# 50001, for 30 exp./~50 sections)
SAP 51841065 (892,50€)

• Nanostring nCounter XT Panels

https://www.nanostring.com/products/PanelPro
1 CodeSet with 1 cartridge can measure in 12 samples up to 770 genes
~1500-3000€ (ms/hu)